Department of Chemistry
Peptidomics study of anthocyanin-rich juice of elderberry
© 2014 Elsevier B.V. All rights reserved. Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper, we describe a method utilizing solid phase extraction (SPE) to remove anthocyanins from peptides. Liquid chromatography coupled with tandem mass spectrometry was used to separate and identify the peptides. The results showed that the use of SPE was an effective method to separate peptides from anthocyanins and other background compounds including high polyphenol content in the juice samples. More than 1000 peptides present in elderberry juice were successfully identified.
Anthocyanin sambucus, Elderberry, Orbitrap-MS/MS, Peptidomics, Solid phase extraction
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Link to Publisher's Edition
Wu, Hanzhi, Mitch C. Johnson, Chi-Hua Lu, Kevin L. Fritsche, Andrew L. Thomas, Yongquan Lai, Zongwei Cai, and C. Michael Greenlief. "Peptidomics study of anthocyanin-rich juice of elderberry." Talanta 131 (2015): 640-644.