Document Type
Journal Article
Department/Unit
Department of Chemistry
Title
Fatty acid profiles reveal toxic responses in adipose tissue of C57BL/6J mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin
Language
English
Abstract
© the Partner Organisations 2014. Dioxin exposure tends to accumulate in adipose tissue and alters metabolism in mammals. In this study, gas chromatography coupled with mass spectrometry (GC-MS) in conjunction with multivariate statistical analysis was applied to profile small molecular metabolites in adipose tissue of aryl hydrocarbon receptor (AhR)-high affinity wild-type C57BL/6J mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). A partial least squares discriminant analysis model was also constructed to map the discrimination between TCDD dosages and the control group. As a result, a total of 16 differential metabolites were identified in the high-dose TCDD group compared to the control group, and 12 free fatty acids (FFAs) were highlighted among them. Both saturated and unsaturated FFA levels were significantly elevated in adipose tissues of TCDD-exposed mice. This promising tool for global characterization highlights FFAs which could be served as indicators for understanding the toxic responses to TCDD exposure in a dose-dependent manner. The data indicated that the use of GC-MS coupled with multivariate statistical analysis could provide new insight for fatty acid biosynthesis on AhR activation with TCDD exposure in wild-type mice.
Publication Date
2014
Source Publication Title
Analytical Methods
Volume
6
Issue
20
Start Page
8207
End Page
8211
Publisher
Royal Society of Chemistry
DOI
10.1039/c4ay01479k
Link to Publisher's Edition
ISSN (print)
17599660
ISSN (electronic)
17599679
Recommended Citation
Hu, Chuanqin, Shuhai Lin, and Zongwei Cai. "Fatty acid profiles reveal toxic responses in adipose tissue of C57BL/6J mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin." Analytical Methods 6.20 (2014): 8207-8211.