School of Chinese Medicine
Background: Dioscorea is a genus of flowering plants, and some Dioscorea species are known and used as a source for the steroidal sapogenin diosgenin. To screen potential resource from Dioscorea species and related medicinal plants for diosgenin extraction, a rapid method to compare the contents of diosgenin in various plants is crucial. Results: An ultra-performance liquid chromatography (UPLC) coupled with diode array detection (DAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for identification and determination of diosgenin in various plants. A comprehensive validation of the developed method was conducted. Twenty-four batches of plant samples from four Dioscorea species, one Smilax species and two Heterosmilax species were analyzed by using the developed method.The present method presented good sensitivity, precision and accuracy. Diosgenin was found in three Dioscorea species and one Heterosmilax species, namely D. zingiberensis, D. septemloba, D. collettii and H. yunnanensis. Conclusion: The method is suitable for the screening of diosgenin resources from plants. D. zingiberensis is an important resource for diosgenin harvesting.
Dioscorea, Diosgenin, Medicinal plants, Quality evaluation, UPLC-DAD-MS
Source Publication Title
© Yi et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
This research was funded by the Faculty Research Grant of Hong Kong Baptist University (FRG2/13-14/031 and FRG1/12-13/035), the Health Department of Guangxi province (Zhong2010111 and Z2010175) and Guangxi Administration of Traditional Chinese Medicine (GZKZ1131) of China.
Link to Publisher's Edition
Yi, Tao, Lan-Lan Fan, Hong-Li Chen, Guo-Yuan Zhu, Hau-Man Suen, Yi-Na Tang, Lin Zhu, Chu Chu, Zhong-Zhen Zhao, and Hu-Biao Chen. "Comparative analysis of diosgenin in Dioscorea species and related medicinal plants by UPLC-DAD-MS." BMC Biochemistry 15 (2014): 19.