Department of Chemistry
Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe
© The Royal Society of Chemistry. A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex 9, [Ir(phq)2(phen)]PF6 (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity. This journal is
Source Publication Title
Royal Society of Chemistry
Link to Publisher's Edition
Leung, Ka-Ho, Hong-Zhang He, Bingyong He, Hai-Jing Zhong, Sheng Lin, Yi-Tao Wang, Dik-Lung Ma, and Chung-Hang Leung. "Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe." Chemical Science 6.4 (2015): 2166-2171.