School of Chinese Medicine; Department of Mathematics
Schisandrin B (SchB) is one of the most abundant bioactive dibenzocyclooctadiene derivatives found in the fruit of Schisandra chinensis. Here, we investigated the potential therapeutic effects of SchB on non-alcoholic fatty-liver disease (NAFLD). In lipidomic study, ingenuity pathway analysis highlighted palmitate biosynthesis metabolic pathway in the liver samples of SchB-treated high-fat-diet-fed mice. Further experiments showed that the SchB treatment reduced expression and activity of fatty acid synthase, expressions of hepatic mature sterol regulatory element binding protein-1 and tumor necrosis factor-α, and hepatic level of palmitic acid which is known to promote progression of steatosis to steatohepatitis. Furthermore, the treatment also reduced hepatic fibrosis, activated nuclear factor-erythroid-2-related factor-2 which is known to attenuate the progression of NASH-related fibrosis. Interestingly, in fasting mice, a single high-dose SchB induced transient lipolysis and increased the expressions of adipose triglyceride lipase and phospho-hormone sensitive lipase. The treatment also increased plasma cholesterol levels and 3-hydroxy-3-methylglutaryl-CoA reductase activity, reduced the hepatic low-density-lipoprotein receptor expression in these mice. Our data not only suggest SchB is a potential therapeutic agent for NAFLD, but also provided important information for a safe consumption of SchB because SchB overdosed under fasting condition will have adverse effects on lipid metabolism.
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This work was partially supported by GRF grants HKBU262512 and HKBU260613 from Research Grant Council of Hong Kong, FRG2/13-14/016, FRG1/13-14/062, FRG2/12-13/039 and FRG2/13-14/030 from Hong Kong Baptist University, JCYJ20120829154222473 from Shenzhen STIC and No.31071989 from the National Natural Science Foundation of China.
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Kwan, H., Niu, X., Dai, W., Tong, T., Chao, X., Su, T., Chan, C., Lee, K., Fu, X., Yi, H., Yu, H., Li, T., Tse, A., Fong, W., Pan, S., Lu, A., & Yu, Z. (2015). Lipidomic-based investigation into the regulatory effect of Schisandrin B on palmitic acid level in non-alcoholic steatotic livers. Scientific Reports, 5, 9114. https://doi.org/10.1038/srep09114