Department of Chemistry
Nanoparticle-encapsulated chlorhexidine against oral bacterial biofilms
Background: Chlorhexidine (CHX) is a widely used antimicrobial agent in dentistry. Herein, we report the synthesis of a novel mesoporous silica nanoparticle-encapsulated pure CHX (Nano-CHX), and its mechanical profile and antimicrobial properties against oral biofilms. Methodology/Principal Findings: The release of CHX from the Nano-CHX was characterized by UV/visible absorption spectroscopy. The antimicrobial properties of Nano-CHX were evaluated in both planktonic and biofilm modes of representative oral pathogenic bacteria. The Nano-CHX demonstrated potent antibacterial effects on planktonic bacteria and mono-species biofilms at the concentrations of 50-200 μg/mL against Streptococcus mutans , Streptococcus sobrinus, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Enterococccus faecalis. Moreover, Nano-CHX effectively suppressed multi-species biofilms such as S. mutans, F. nucleatum, A. actinomycetemcomitans and Porphyromonas gingivalis up to 72 h. Conclusions/Significance: This pioneering study demonstrates the potent antibacterial effects of the Nano-CHX on oral biofilms, and it may be developed as a novel and promising anti-biofilm agent for clinical use. © 2014 Seneviratne et al.
Source Publication Title
Public Library of Science
Link to Publisher's Edition
Seneviratne, C., Leung, K., Wong, C., Lee, S., Li, X., Leung, P., Lau, C., Wat, E., & Jin, L. (2014). Nanoparticle-encapsulated chlorhexidine against oral bacterial biofilms. PLoS ONE, 9 (8), e103234. https://doi.org/10.1371/journal.pone.0103234