Document Type
Journal Article
Department/Unit
School of Chinese Medicine
Title
Tissue-specific metabolite profiling of Turmeric by using laser micro-dissection, ultra-high performance liquid chromatography-quadrupole time of fight-mass spectrometry and liquid chromatography-tandem mass spectrometry
Language
English
Abstract
© IM Publications LLP 2014 All rights reserved. Curcuma longa L. is recognized for its therapeutic and culinary uses both in Ayurveda and traditional Chinese medicine and is considered to be a boon to mankind. It has been extensively studied for its benefits and still continues to be an important drug with continued potential for further exploration and research. We studied the tissue-specific distribution of secondary metabolites to establish the validity of the use of rhizome samples from India and China, as substitutes for each other, based upon their metabolite profiles and curcumin contents. Laser microdissection was used for the isolation of microscopic tissues, such as cork, cortex and leaf-trace vascular bundles from rhizomes. Metabolite profiling was carried out by ultra-high performance liquid chromatography-quadrupole time of fight-mass spectrometry and curcumin content was estimated by a method validated as per the Harmonized Tripartite Guidelines. The cortex and cork revealed the presence of a higher number of secondary metabolites than in the leaf-trace vascular bundles. The curcumin contents in rhizome samples from both the countries, estimated with the help of a precise and accurate validated method, were found to be comparable. Based on the results, we conclude that turmeric rhizomes grown in India and China are qualitatively and quantitatively indistinguishable and therefore can be used as substitutes. The developed method can be widely applied for microscopic identification, authentication and analysis of the distribution of phytoconstituents in other botanical species of interest or of species with a significant commercial and therapeutic value.
Keywords
Curcuma longa l., Curcumin, LC-MS/MS, LMD, Metabolite profiling, UHPLC-QTOF-MS
Publication Date
2014
Source Publication Title
European Journal of Mass Spectrometry
Volume
20
Issue
5
Start Page
383
End Page
393
Publisher
IM Publications
DOI
10.1255/ejms.1295
Link to Publisher's Edition
ISSN (print)
14690667
ISSN (electronic)
17516838
Recommended Citation
Jaiswal, Yogini, Zhitao Liang, Alan Ho, Hubiao Chen, and Zhongzhen Zhao. "Tissue-specific metabolite profiling of Turmeric by using laser micro-dissection, ultra-high performance liquid chromatography-quadrupole time of fight-mass spectrometry and liquid chromatography-tandem mass spectrometry." European Journal of Mass Spectrometry 20.5 (2014): 383-393.