Department of Chemistry
A G-quadruplex-selective luminescent probe with an anchor tail for the switch-on detection of thymine DNA glycosylase activity
© 2016 Elsevier B.V. Thymine DNA glycosylase (TDG) performs essential functions in maintaining genetic integrity and epigenetic regulation, which also plays an essential role in DNA demethylation. In this work, the novel iridium(III) complex 1 with an anchor tail was synthesized and employed to construct a G-quadruplex-based assay for detecting TDG activity in aqueous solution by using the mismatched base excising property of TDG with T4 DNA ligase and phi29 DNA polymerase, in concert with the rolling circle amplification (RCA) strategy. The assay achieved a detection limit of 0.048 U mL− 1 (0.012 ng mL−1), and showed high selectivity towards TDG even in the presence of other proteins and enzymes. Additionally, the assay could function in diluted cellular debris.
G-quadruplex, Iridium(III) complex, Luminescence, TDG
Source Publication Title
Biosensors and Bioelectronics
Link to Publisher's Edition
Lin, Sheng, Tian-Shu Kang, Lihua Lu, Wanhe Wang, Dik-Lung Ma, and Chung-Hang Leung. "A G-quadruplex-selective luminescent probe with an anchor tail for the switch-on detection of thymine DNA glycosylase activity." Biosensors and Bioelectronics 86 (2016): 849-857.