Document Type
Journal Article
Department/Unit
Department of Chemistry
Title
A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3′–5′ proofreading activity
Language
English
Abstract
We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes. © 2013 Elsevier Inc.
Keywords
G-quadruplex, Iridium(III) complex, Luminescent, Polymerase, Proofreading
Publication Date
2013
Source Publication Title
Methods
Volume
64
Issue
3
Start Page
224
End Page
228
Publisher
Elsevier
DOI
10.1016/j.ymeth.2013.05.017
Link to Publisher's Edition
ISSN (print)
10462023
ISSN (electronic)
10959130
Recommended Citation
Leung, Ka-Ho, Hong-Zhang He, Hai-Jing Zhong, Lihua Lu, Daniel Shiu-Hin Chan, Dik-Lung Ma, and Chung-Hang Leung. "A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3′–5′ proofreading activity." Methods 64.3 (2013): 224-228.