Department of Chemistry
A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3′–5′ proofreading activity
We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes. © 2013 Elsevier Inc.
G-quadruplex, Iridium(III) complex, Luminescent, Polymerase, Proofreading
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Leung, Ka-Ho, Hong-Zhang He, Hai-Jing Zhong, Lihua Lu, Daniel Shiu-Hin Chan, Dik-Lung Ma, and Chung-Hang Leung. "A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3′–5′ proofreading activity." Methods 64.3 (2013): 224-228.