Department of Biology
A quinolinyl antipyrine based fluorescence sensor for Zn2+ and its application in bioimaging
By incorporating 4-aminoantipyrine moiety onto 8-aminoquinoline with a suitable spacer, a highly selective and sensitive fluorescent Zn2+ sensor, QPA, was designed and constructed. In 25% ACN-HEPES buffer pH 7.0 solution, QPA exhibited 10.6-fold fluorescence enhancement at 500 nm upon addition of Zn2+. The limit of detection (LOD) was calculated to be 1.3 × 10-7 M according to fluorescence titration. The 1:1 binding mode of the metal complex was established by combined UV-vis, fluorescence and HRMS spectroscopic method. The membrane permeability of QPA to living cells and bioimaging of Zn2+ are demonstrated. © 2012 The Royal Society of Chemistry.
Source Publication Title
Royal Society of Chemistry
Link to Publisher's Edition
You, Q., Chan, P., Chan, W., Hau, S., Lee, A., Mak, N., Mak, T., & Wong, R. (2012). A quinolinyl antipyrine based fluorescence sensor for Zn2+ and its application in bioimaging. RSC Advances, 2 (29), 11078-11083. https://doi.org/10.1039/C2RA21736H