Department of Biology
A quinolinyl antipyrine based fluorescence sensor for Zn2+ and its application in bioimaging
By incorporating 4-aminoantipyrine moiety onto 8-aminoquinoline with a suitable spacer, a highly selective and sensitive fluorescent Zn2+ sensor, QPA, was designed and constructed. In 25% ACN-HEPES buffer pH 7.0 solution, QPA exhibited 10.6-fold fluorescence enhancement at 500 nm upon addition of Zn2+. The limit of detection (LOD) was calculated to be 1.3 × 10-7 M according to fluorescence titration. The 1:1 binding mode of the metal complex was established by combined UV-vis, fluorescence and HRMS spectroscopic method. The membrane permeability of QPA to living cells and bioimaging of Zn2+ are demonstrated. © 2012 The Royal Society of Chemistry.
Source Publication Title
Royal Society of Chemistry
Link to Publisher's Edition
You, Qi-Hua, Pui-Shan Chan, Wing-Hong Chan, Sam C.K. Hau, Albert W.M. Lee, N. K. Mak, Thomas C.W. Mak, and Ricky N.S. Wong. "A quinolinyl antipyrine based fluorescence sensor for Zn2+ and its application in bioimaging." RSC Advances 2.29 (2012): 11078-11083.