Document Type
Journal Article
Department/Unit
Department of Chemistry
Title
Label-free luminescent oligonucleotide-based probes
Language
English
Abstract
Breakthrough advances in chemistry and biology over the last two decades have vastly expanded the repertoire of nucleic acid structure and function with potential application in multiple areas of science and technology, including sensing and analytical applications. DNA oligonucleotides represent popular tools for the development of sensing platforms due to their low cost, rich structural polymorphism, and their ability to bind to cognate ligands with sensitivity and specificity rivaling those for protein enzymes and antibodies. In this review, we give an overview of the "label-free" approach that has been a particular focus of our group and others for the construction of luminescent DNA-based sensing platforms. The label-free strategy aims to overcome some of the drawbacks associated with the use of covalently-labeled oligonucleotides prevalent in electrochemical and optical platforms. Label-free DNA-based probes harness the selective interaction between luminescent dyes and functional oligonucleotides that exhibit a "structure-switching" response upon binding to analytes. Based on the numerous examples of label-free luminescent DNA-based probes reported recently, we envisage that this field would continue to thrive and mature in the years to come. © 2013 The Royal Society of Chemistry.
Publication Date
2013
Source Publication Title
Chemical Society Reviews
Volume
42
Issue
8
Start Page
3427
End Page
3440
Publisher
Royal Society of Chemistry
DOI
10.1039/C2CS35472A
Link to Publisher's Edition
ISSN (print)
03060012
ISSN (electronic)
14604744
Recommended Citation
Ma, Dik-Lung, Hong-Zhang He, Ka-Ho Leung, Hai-Jing Zhong, Daniel Shiu-Hin Chan, and Chung-Hang Leung. "Label-free luminescent oligonucleotide-based probes." Chemical Society Reviews 42.8 (2013): 3427-3440.