Department of Chemistry
L-Ascorbic acid biosensing assay from enzyme-immobilized pig bladder membrane as a novel platform
An l-ascorbic acid biosensing assay was developed using the enzyme-immobilized pig bladder membrane and dissolved oxygen electrode. The ascorbate oxidase immobilized with chitosan was bound to bladder membrane by glutaraldehyde as a crosslinking agent. The phosphate buffer (100 mM, pH = 5.0), glutaraldehyde concentration (15%), 0.035 mg enzyme loading, and 20-30 °C were established as the optimum test conditions. This biosensor exhibited a response time of 80 s, a generous linear range of 0.010 mM to 0.88 mM with a detection limit of 10 μM, repeatability (3.1%, n = 20), recoveries (98.7-102%), and good stability with a shelf-life of more than 3 months. The reproducibility of fabrication of the biosensors was investigated by using three different membranes (R.S.D. = 3.0%). The comparison of the analytical performance between the published and the proposed biosensor methods was made. This biosensor has been applied to determine the l-ascorbic acid content in real samples, and the results are in agreement with those obtained by a commercial colorimetric ascorbic acid assay kit. © 2013 The Royal Society of Chemistry.
Source Publication Title
Royal Society of Chemistry
Link to Publisher's Edition
Li, Fengxia, Hongjuan Wu, Miao Cui, Yan Zhang, Chuan Dong, Junping Wang, Shaomin Shuang, and Martin M. F. Choi. "L-Ascorbic acid biosensing assay from enzyme-immobilized pig bladder membrane as a novel platform." Analytical Methods 5.5 (2013): 1253-1258.