Document Type
Journal Article
Department/Unit
Department of Chemistry
Title
N-phosphorylation labeling for analysis of twenty natural amino acids and small peptides by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Language
English
Abstract
N-phosphorylation labeling was utilized to analyze the low molecular weight compounds by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A wide range of natural amino acids and short peptides was successfully analyzed by MALDI-TOF MS without matrix background interferences. The N-phosphorylation labeling reaction was carried out easily within 30 min in a one-pot reaction under mild reaction conditions. The phosphoryl derivatization reaction is a global labeling approach with high selectivity and high specificity with targeting only on the N-terminal and ε-amino group of Lys. The incorporation of a neutral phosphoryl group with high gas-phase affinity of protons not only improves the ionization efficiency of target molecules and simultaneously decreases the ion suppression effects in MALDI-TOF MS analysis, but also greatly reduces or eliminates the matrix background interferences by suppressing the matrix signals and increasing the molecular weight of the targeted compounds. By applying the N-phosphorylation labeling approach, many amino acids could be detected in serum samples by using MALDI-TOF MS. © 2013 The Royal Society of Chemistry.
Publication Date
2013
Source Publication Title
Analyst
Volume
138
Issue
9
Start Page
2632
End Page
2639
Publisher
Royal Society of Chemistry
DOI
10.1039/c3an00036b
Link to Publisher's Edition
http://dx.doi.org/10.1039/c3an00036b
ISSN (print)
00032654
ISSN (electronic)
13645528
APA Citation
Gao, X., Bi, X., Wei, J., Peng, Z., Liu, H., Jiang, Y., Wei, W., & Cai, Z. (2013). N-phosphorylation labeling for analysis of twenty natural amino acids and small peptides by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Analyst, 138 (9), 2632-2639. https://doi.org/10.1039/c3an00036b