School of Chinese Medicine
Litsea verticillata Hance (Lauraceae), a Chinese medicine used to treat swelling caused by injury or by snake bites, was the first plant identified by our National Institutes of Health (NIH)-funded International Cooperative Biodiversity Group (ICBG) project to exhibit anti-HIV activities. From this plant, we discovered a class of 8 novel litseane compounds, prototypic sesquiterpenes, all of which demonstrated anti-HIV activities. In subsequent studies, 26 additional compounds of different structural types were identified. During our continuing investigation of this plant species, we identified two new litseanes, litseaverticillols L and M, and a new sesquiterpene butenolide, litseasesquibutenolide. Litseaverticillols L and M were found to inhibit HIV-1 replication, with an IC[Formula: see text] value of 49.6[Formula: see text][Formula: see text]M. To further determine the antiviral properties of this plant, several relatively abundant isolates, including a litseane compound, two eudesmane sesquiterpenes and three lignans, were evaluated against an additional 21 viral targets. Lignans 8 and 9 were shown to be active against the Epstein-Barr Virus (EBV), with EC[Formula: see text] values of 22.0[Formula: see text][Formula: see text]M ([Formula: see text]) and 16.2[Formula: see text][Formula: see text]M ([Formula: see text]), respectively. Since many antiviral compounds have been discovered in L. verticillata, we further prepared 38 plant extracts made from the different plant parts of 9 additional Litsea species. These extracts were evaluated for their anti-HIV and cytotoxic activities, and four of the extracts, which ranged across three different species, displayed 97-100% inhibitory effects against HIV replication without showing cytotoxicity to a panel of human cell lines at a concentration of 20 μg/mL.
Litsea, Lauraceae, Litseane, Sesquiterpene Butenolide, Antiviral Activity, AntiHIV Activity, Structure Determination
Source Publication Title
American Journal of Chinese Medicine
World Scientific Publishing
Electronic version of an article published as American Journal of Chinese Medicine ,Volume 44, Issue 2, Year 2016, Page 275-290, 10.1142/S0192415X16500166 © copyright World Scientific Publishing Company http://dx.doi.org/10.1142/S0192415X16500166
The work described in this paper was supported by NIH Grants 3U01TW001015-10S1 and 2U01TW001015-11A1 (administered by the Fogarty International Center as part of an International Cooperative Biodiversity Groups program, through funds from NIH, NSF, and Foreign Agricultural Service of the USDA), the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. HKBU 262912), HKBU Interdisciplinary Research Matching Scheme (RC-IRMS/12-13/03), Faculty Research Grant, Hong Kong Baptist University (FRG1/13-14/029), National Natural Science Foundation of China (Grant No. 21402166) and Mr. Kwok Yat Wai and Madam Kwok Chung Bo Fun Graduate School Development Fund.
Link to Publisher's Edition
Guan, Yifu, Dongying Wang, Ghee T. Tan, Nguyen Van Hung, Nguyen Manh Cuong, John M. Pezzuto, Harry H. S. Fong, Djaja Doel Soejarto, and Hongjie Zhang. "Litsea species as potential antiviral plant sources." American Journal of Chinese Medicine 44.2 (2016): 275-290.