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Journal Article


School of Chinese Medicine




This paper reports a novel strategy based on high-speed counter-current chromatography (HSCCC) technique to separate in vivo metabolites from refined extract of urine after administration of an herbal medicine. Saussurea laniceps (SL) was chosen as a model herbal medicine to be used to test the feasibility of our proposed strategy. This strategy succeeded in the case of separating four in vivo metabolites of SL from the urine of rats. Briefly, after oral administration of SL extract to three rats for ten days (2.0 g/kg/d), 269.1 mg of umbelliferone glucuronide (M1, purity, 92.5%), 432.5 mg of scopoletin glucuronide (M2, purity, 93.2%), 221.4 mg of scopoletin glucuronide (M3, purity, 92.9%) and 319.0 mg of scopoletin glucuronide (M4, purity, 90.4%) were separated from 420 mL of the rat urine by HSCCC using a two-phase solvent system composed of methyl tert-butyl ether–n-butanol–acetonitrile–water (MTBE–n-BuOH–ACN–H2O) at a volume ratio of 10:30:11:49. The chemical structures of the four metabolites, M1 to M4,were confirmed by MS and 1H, 13C NMR. As far as we know, this is the first report of the successful separation of in vivo metabolites by HSCCC after administration of an herbal medicine.

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Scientific Reports



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Article number: 33036


Nature Publishing Group

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This work was partially supported by the Faculty Research Grant of Hong Kong Baptist University (FRG2/14-15/061) the Innovation and Technology Fund of Hong Kong (ITS/280/15), the National Natural Science Foundation of China (81673691) and the Guandong Natural Science Foundation (2014A030313766).



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JA-5173-29261_suppl.pdf (861 kB)
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JA-5173-29261_suppl.pdf (861 kB)
Supplementary information