School of Chinese Medicine
Rapid and sensitive determination of the major steroidal saponins of Ypsilandra thibetica Franch by ultra high-performance liquid chromatography coupled with triple quadrupole mass spectrometry
A rapid and validated method using ultra high-performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UHPLC-QQQ MS) was developed for simultaneous determination of four active steroidal saponins, i.e., dichotomin (1), pennogenin 3-O-α-L-arabinofuranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (2), pennogenin 3-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside (3) and diosgenin 3-O-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranosidein (4), in Ypsilandra thibetica Franch. The optimized sample preparation and UHPLC-QQQ MS conditions were chosen for quantitative analysis. The separation was performed on an Agilent Zorbax Eclipse Plus C18 column (2.1 mm × 50 mm, 1.8 µm) with gradient elution of acetonitrile–0.1% formic acid in water. All calibration curves showed good linear regression (r> 0.9985) within the test range. The limits of detection and quantification were in the range of 0.02–4.40 and 0.04–22.0 ng/mL, respectively. The proposed method was applied to analyze two batches of Y. thibetica samples for target compounds within 10 min. This work promoted the quality control method for raw material or preparations of Y. thibetica.
Source Publication Title
Journal of Chromatographic Science
Oxford University Press
This project was supported by the Training Program for Outstanding Young Teachers in Higher Education Institutions of Guangdong Province in 2015 (No. YQ2015196), Natural Science Fund Project of Guangdong Province (No. S2012040007924) and Medical Science Fund Project of Guangdong Province (No. B2012058).
Link to Publisher's Edition
Xia, L., Quyang, P., Gao, W., Yi, T., Zhang, X., Zhao, Z., & Yang, H. (2016). Rapid and sensitive determination of the major steroidal saponins of Ypsilandra thibetica Franch by ultra high-performance liquid chromatography coupled with triple quadrupole mass spectrometry. Journal of Chromatographic Science, 54 (6), 1010-1015. https://doi.org/10.1093/chromsci/bmw037