Document Type
Journal Article
Department/Unit
School of Chinese Medicine
Language
English
Abstract
Cell type-specific metabolite analysis is a promising method for understanding plant metabolite production, function, transport and storage. In the present study, laser microdissection (LMD) and ultra-high performance liquid chromatography quadrupole/time of flight-mass spectrometry are combined to determine where secondary metabolites are accumulated in the roots of Bupleurum scorzonerifolium Willd, Bupleurum chinense DC. and Bupleurum falcatum L. Four tissues, namely cork, cortex, phloem and xylem, were microdissected by laser microdissection, and their chemical profiles were analyzed. The main metabolites are saikosaponins. Different tissues contained different saikosaponins. Generally, the cork and cortex from all three species contained more types of saikosaponins and higher contents of saikosaponins a, c and d than did the phloem and xylem. Interestingly, in the roots of Bupleurum scorzonerifolium and B. falcatum, the cork contained much higher contents of saikosaponins a, c and d than did the cortex; while in the root of B. chinense, the cortex contained higher contents of saikosaponins a, c and d than the cork. Explanation and application of the results are discussed. The present findings yield valuable insights into the quality evaluation of Bupleuri Radix by morphological features.
Keywords
Bupleurum, Tissue specific, Metabolite profiling, Laser microdissection, UHPLC–QTOF-MS
Publication Date
8-2014
Source Publication Title
Journal of Pharmaceutical and Biomedical Analysis
Volume
97
Start Page
157
End Page
165
Publisher
Elsevier
Peer Reviewed
1
Copyright
Copyright © 2014 Elsevier B.V. All rights reserved.
Funder
This work is supported by the National Natural Science Foundation of the People's Republic of China (project no. 81303219).
DOI
10.1016/j.jpba.2014.04.033
Link to Publisher's Edition
http://dx.doi.org/10.1016/j.jpba.2014.04.033
ISSN (print)
07317085
ISSN (electronic)
1873264X
Recommended Citation
Liang, Zhitao, Kayan Oh, Yuqing Wang, Tao Yi, Hubiao Chen, and Zhongzhen Zhao. "Cell type-specific qualitative and quantitative analysis of saikosaponins in three Bupleurum species using laser microdissection and liquid chromatography–quadrupole/time of flight-mass spectrometry." Journal of Pharmaceutical and Biomedical Analysis 97 (2014): 157-165.
Appendix A. Supplementary data