School of Chinese Medicine
Anti-inflammatory activity of the extracts and fractions from Erigeron multiradiatus through bioassay-guided procedures
Erigeron multiradiatus (Lindl.) Benth., an herb that grows in the alpine and subalpine meadow of Qinghai-Tibet plateau, has been widely used as a folk remedy by the native people for treatment of various inflammatory ailments.
Aim of the study
In order to isolate and identify the active components of Erigeron multiradiatus for anti-inflammatory activity, a preliminary phytochemical study and a bioassay-guided fractionation and purification process was performed.
Material and Methods
The dry whole plant Erigeron multiradiatus was extracted with 50% ethanol and then separated into CHCl3, n-BuOH, and aqueous fractions. The anti-inflammatory activities of each fraction were investigated using two in vivo inflammation models.
These results exhibited varying degrees of anti-inflammatory activities and the n-BuOH fraction showed the strongest anti-inflammatory activities. The n-BuOH fraction was then subjected to separation and purification using macroporous resins column chromatography and Sephadex LH-20 leading to two flavonoids glucuronides identified as scutellarein-7-O-β-glucuronide and apigenin-7-O-β-glucuronide. Furthermore, LC–MS/MS identification and quantification of isolated compounds were also performed.
Scutellarein-7-O-β-glucuronide and apigenin-7-O-β-glucuronide were considered as major components and principally responsible for the anti-inflammatory activity of Erigeron multiradiatus. Thus the results of our study provide a scientific basis for the utilization of Erigeron multiradiatus in traditional Tibetan medicine.
Erigeron multiradiatus, Anti-inflammatory activity, Flavonoids, LC–MS/MS
Source Publication Title
Journal of Ethnopharmacology
The authors thank the Bureau of Science and Technology of Sichuan Province for the support of the applied fundamental study (No. 2006Z08—081). Thanks are also due to The Ministry of National Education Doctoral Fund (No. 20020610089) for providing financial support.
Link to Publisher's Edition
Luo, Pei, Zhifeng Zhang, Tao Yi, Hao Zhang, Xinfu Liu, and Zhengji Mo. "Anti-inflammatory activity of the extracts and fractions from Erigeron multiradiatus through bioassay-guided procedures." Journal of Ethnopharmacology 119.2 (2008): 232-239.