School of Chinese Medicine
A new procedure for the separation and isolation of Z-ligustilide from the roots of Angelica sinensis (AS) was developed, and the storage conditions for Z-ligustilide were optimized. Using the present procedure, Z-ligustilide was enriched by decomposing theZ-ligustilide dimers yielding Z-ligustilide and dissolving the polar impurities in hot water. Then, the crude Z-ligustilide was further purified by a semipreparative HPLC system. The spiked and nonspiked samples were used for the evaluation of the proposed procedure. Recoveries obtained varied from 86.2 to 90.7% and RSDs from 4.0 to 6.6%. The yield and purity of the isolated Z-ligustilide were found to be 4.57 mg/g and 99.6%, respectively. The results of stability tests have shown that the presence of oxidant contributes to Z-ligustilide degradation, therefore argon was chosen as a shielding gas for storage. The overall procedure is efficient and convenient which is considered suitable for the preparative separation of Z-ligustilide from AS.
Angelica sinensis, Semipreparative HPLC, Shielding gas, Z-Ligustilide
Source Publication Title
Journal of Separation Science
This is the peer reviewed version of the following article: Yi, T., Zhang, H., Xie, J. and Xue, D. (2007), A new procedure for the preparative separation and isolation of Z-ligustilide from the roots of Angelica sinensis. J. Sep. Science, 30: 1973–1978. doi:10.1002/jssc.200700001, which has been published in final form at http://dx.doi.org/10.1002/jssc.200700001 article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Link to Publisher's Edition
Yi, Tao, Hao Zhang, Jing Xie, and Dan Xue. "A new procedure for the preparative separation and isolation of Z-ligustilide from the roots of Angelica sinensis." Journal of Separation Science 30.12 (2007): 1973-1978.