Document Type

Journal Article

Department/Unit

Department of Chemistry

Language

English

Abstract

Early cancer diagnosis is critically important for early intervention and it can significantly enhance the treatment efficacy and the chance of cure. Herein, for the first time, the cyanine fluorophore namely, SLSO3, was synthesized and found to possess a high binding affinity towards a typical biomarker, carcinoembryonic antigen (CEA) in which it served as an excellent turn-on labelling fluorophore for the detection of CEA. On this basis, the target CEA antigen is captured by a primary antibody conjugated on magnetic silica coated iron oxide nanoparticles and then followed by secondary antibody-loaded SiO2 nanoparticles, which provide more binding sites for the labelling dyes resulting in 6-fold increase in fluorescence and thus heightened sensitivity. The immunosensor is capable of differentiating target analyte from other cancer-associated proteins and has been applied to quantify CEA in human serum sample and the result was consistent to that obtained from a commercial ELISA kit. This simple and direct detection assay has achieved a low limit of detection in the pg mL−1 regime with small sample consumption (only 10 μL) and without any sample pre-treatment and purification.

Keywords

Immunosensor, Turn-on fluorophore, Cancer biomarker, Carcinoembryonic antigen, Signal amplification

Publication Date

4-1-2018

Source Publication Title

Sensors and Actuators B: Chemical

Volume

258

Start Page

133

End Page

140

Publisher

Elsevier

Peer Reviewed

1

Copyright

© 2017 Elsevier B.V. All rights reserved.

Funder

We are thankful to the financial support of National Natural Science Foundation of China (21405063 and 21675135). We also thank the support of the Research Grant Council of Hong Kong (12308416 and C2012-15G).

DOI

10.1016/j.snb.2017.11.103

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.snb.2017.11.103

ISSN (print)

09254005

Available for download on Friday, May 01, 2020

Included in

Chemistry Commons

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