Document Type
Journal Article
Department/Unit
Department of Chemistry
Title
A new method for the analysis of β2-agonists in human urine by pressure-assisted capillary electrochromatography coupled with electrospray ionization-mass spectrometry using a silica-based monolithic column
Language
English
Abstract
A new pressure-assisted capillary electrochromatography coupled with electrospray ionization-mass spectrometry method using a silica-based monolithic column as separation media was developed for the analysis of β2-agonists in human urine. Experimental conditions including the mobile phase, separation voltage, assisted pressure, and sheath liquid were optimized for the analysis: mobile phase composed of 82% (v/v) ACN and 18% (v/v) 20 mmol/L ammonium acetate (pH 6.0); separation voltage 25 kV; assisted pressure 2 bar; and the sheath liquid consisting of 7.5 mmol/L acetic acid in isopropanol/water 50/50% (v/v) that was delivered at a flow rate of 3.0 μL/min. Six β2-agonists were separated within 12.5 min with LODs (defined as S/N = 3) in the range of 0.25-2.0 ng/mL. The absolute LODs of the developed method for analyzing six β2-agonists ranged from 5.75 to 46.0 fg. Method repeatability of run-to-run and column-to-column was satisfactory. The recovery obtained from the analysis of spiked urine samples was between 88.2% and 106% with RSDs lower than 6.68%. The method was successfully applied to the analysis of real urine sample from volunteers. © 2010 Elsevier B.V. All rights reserved.
Keywords
β2-Agonists, Capillary electrochromatography, Electrospray ionization-mass spectrometry, Silica-based monolithic column
Publication Date
2010
Source Publication Title
Talanta
Volume
81
Issue
5-4
Start Page
1655
End Page
1661
Publisher
Elsevier
DOI
10.1016/j.talanta.2010.03.020
Link to Publisher's Edition
http://dx.doi.org/10.1016/j.talanta.2010.03.020
ISSN (print)
00399140
ISSN (electronic)
18733573
APA Citation
Lu, M., Zhang, L., Li, X., Lu, Q., Chen, G., & Cai, Z. (2010). A new method for the analysis of β2-agonists in human urine by pressure-assisted capillary electrochromatography coupled with electrospray ionization-mass spectrometry using a silica-based monolithic column. Talanta, 81 (5-4), 1655-1661. https://doi.org/10.1016/j.talanta.2010.03.020