Document Type
Journal Article
Department/Unit
Department of Chemistry
Title
Analysis of adenosine phosphates in HepG-2 cell by a HPLC–ESI-MS system with porous graphitic carbon as stationary phase
Language
English
Abstract
A high performance liquid chromatography coupled with electrospray ionization/mass spectrometry method was developed for the determination of adenosine 5′-monophosphate (AMP), adenosine 5′-diphosphate (ADP), and adenosine 5′-triphosphate (ATP) in the extract of HepG-2 cells. The chromatographic conditions were optimized by using porous graphitic carbon as the stationary phase for the retention and separation of the AMP, ADP and ATP. Negative-ion mode ESI-MS in basic mobile phase was applied to improve the method sensitivity. An external calibration method with linear ranges from 0.22 to 57.80 μM for AMP, from 0.59 to 117.37 μM for ADP, and from 0.49 to 98.81 μM for ATP was used for quantitative analysis. The levels of ATP, ADP, and AMP in HepG-2 cells treated with benzo[a]pyrene with different time periods were determined. Total adenine nucleotides and the energy charge potential were calculated for the investigation of the effect of benzo[a]pyrene on cell energy metabolism. © 2009 Elsevier B.V. All rights reserved.
Keywords
Adenosine nucleotides, Benzo[a]pyrene, HepG-2 cell, LC-ESI-MS, Porous graphitic carbon
Publication Date
2009
Source Publication Title
Journal of Chromatography B
Volume
877
Issue
22
Start Page
2019
End Page
2024
Publisher
Elsevier
DOI
10.1016/j.jchromb.2009.05.027
Link to Publisher's Edition
http://dx.doi.org/10.1016/j.jchromb.2009.05.027
ISSN (print)
15700232
APA Citation
Wang, J., Lin, T., Lai, J., Cai, Z., & Yang, M. (2009). Analysis of adenosine phosphates in HepG-2 cell by a HPLC–ESI-MS system with porous graphitic carbon as stationary phase. Journal of Chromatography B, 877 (22), 2019-2024. https://doi.org/10.1016/j.jchromb.2009.05.027