Department of Chemistry
Isolation of a Methylobacterium organophilium strain, and its application to a methanol biosensor
A methanol-utilizing strain (ME25) using methanol (MeOH) as the sole carbon source has been isolated from methane-generating pits. ME25 was identified as Methylobacterium organophilium by its physiological characteristics and 16SrDNA sequence. A MeOH biosensor was then developed by immobilizing ME25 along with sensor for dissolved oxygen (O2). Its response is based on the depletion of O2 following oxidation of MeOH by the bacteria. The decrease in O2 is linearly related to the MeOH concentration in the range from 1.6 mg·L-1 to 4800 mg·L-1 and the detection limit for MeOH is 0.27 mg·L-1. The response time of the biosensor is around 20 min. © Springer-Verlag 2009.
Dissolved oxygen, Methane-generating pits, Methanol biosensing system, Methanol determination, Methylobacterium organophilium
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Zhao, Chungui, Haipeng Li, Jun Zheng, Liang Chen, Feng Li, Suping Yang, Chuan Dong, and Martin M.F. Choi. "Isolation of a Methylobacterium organophilium strain, and its application to a methanol biosensor." Microchimica Acta 167.2-1 (2009): 67-73.