Department of Chemistry
Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' → 5' exonuclease activity
A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1. © 2010 American Chemical Society.
Source Publication Title
American Chemical Society
Link to Publisher's Edition
Leung, C., Chan, D., Man, B., Wang, C., Lam, W., Cheng, Y., Fong, W., Hsiao, W., & Ma, D. (2011). Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' → 5' exonuclease activity. Analytical Chemistry, 83 (2), 463-466. https://doi.org/10.1021/ac1025896