Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

An ethanol biosensor based on a bacterial cell-immobilized eggshell membrane

Language

English

Abstract

An ethanol biosensor was fabricated based on a Methylobacterium organophilium-immobilized eggshell membrane and an oxygen (O 2) electrode. A linear response for ethanol was obtained in the range of 0.050-7.5 mmol/L with a detection limit of 0.025 mmol/L (S/N = 3) and a R.S.D. of 2.1%. The response time was less than 100 s at room temperature and ambient pressure. The optimal loading of bacterial cells on the biosensor membrane is 40 mg (wet weight). The optimal working conditions for the microbial biosensor are pH 7.0 phosphate buffer (50 mmol/L) at 20-25°C. The interference test, operational and storage stability of the biosensor are studied in detail. Finally, the biosensor is applied to determine the ethanol contents in various alcohol samples and the results are comparable to that obtained by gas chromatographic method and the results are satisfactory. Our proposed biosensor provides a convenient, simple and reliable method to determine ethanol content in alcoholic drinks. © 2012 Chuan Dong. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

Keywords

Eggshell membrane, Ethanol, Methylobacterium organophilium, Microbial biosensor, Oxygen electrode, Wine

Publication Date

2012

Source Publication Title

Chinese Chemical Letters

Volume

23

Issue

4

Start Page

481

End Page

483

Publisher

Elsevier

DOI

10.1016/j.cclet.2012.01.026

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.cclet.2012.01.026

ISSN (print)

10018417

ISSN (electronic)

18785964

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