Department of Chemistry
Application of hydrophobic palladium nanoparticles for the development of electrochemical glucose biosensor
An amperometric glucose biosensor based on an n-alkylamine-stabilized palladium nanoparticles (PdNPs)-glucose oxidase (GOx) modified glassy carbon (GC) electrode has been successfully fabricated. PdNPs were initially synthesized by a biphase mixture of water and toluene method using n-alkylamines (dodecylamine, C 12-NH 2 and octadecylamine, C 18-NH 2) as stabilizing ligands. The performance of the PdNPs-GOx/GC biosensor was studied by cyclic voltammetry. The optimum working potential for amperometric measurement of glucose in pH 7.0 phosphate buffer solution is -0.02V (vs. Ag/AgCl). The analytical performance of the biosensor prepared from C 18-PdNPs-GOx is better than that of C 12-PdNPs-GOx. The C 18-PdNPs-GOx/GC biosensor exhibits a fast response time of ca. 3s, a detection limit of 3.0μM (S/N=3) and a linear range of 3.0μM-8.0mM. The linear dependence of current density with glucose concentration is 70.8μAcm -2mM -1. The biosensor shows good stability, repeatability and reproducibility. It has been successfully applied to determine the glucose content in human blood serum samples. © 2011 Elsevier B.V.
Amperometry, Glucose, Palladium nanoparticles
Source Publication Title
Biosensors and Bioelectronics
Link to Publisher's Edition
Li, Zhongping, Xu Wang, Guangming Wen, Shaomin Shuang, Chuan Dong, Man Chin Paau, and Martin M.F. Choi. "Application of hydrophobic palladium nanoparticles for the development of electrochemical glucose biosensor." Biosensors and Bioelectronics 26.11 (2011): 4619-4623.