Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Preparation and evaluation of a phenylboronate affinity monolith for selective capture of glycoproteins by capillary liquid chromatography

Language

English

Abstract

A phenylboronate affinity monolith was prepared and applied to the selective capture of glycoproteins from unfractionated protein mixtures. The monolith was synthesized in a 100 μm i.d capillary by an in situ polymerization procedure using a pre-polymerization mixture consisting of 4-vinylphenylboronic acid (VPBA) as functional monomer, ethylene dimethacrylate (EDMA) as crosslinker, diethylene glycol and ethylene glycol as binary porogenic solvents, and azobisisobutyronitrile (AIBN) as initiator. The prepared monolith was characterized in terms of the morphology, pore property, and recognition property. The selectivity and dynamic binding capacity were evaluated by using standard glycoproteins and nonglycoproteins as model proteins. The chromatographic results demonstrated that the phenylboronate affinity monolith had higher selectivity and binding capacity for glycoprotein than nonglycoprotein. The resulting phenylboronate affinity monolith was used as the sorbent for in-tube solid phase microextraction (in-tube SPME), and the extraction performance of the monolith was assessed by capture of ovalbumin from egg white sample. © 2011 The Royal Society of Chemistry.

Publication Date

2011

Source Publication Title

Analyst

Volume

136

Issue

16

Start Page

3281

End Page

3288

Publisher

Royal Society of Chemistry

DOI

10.1039/c1an15180k

Link to Publisher's Edition

http://dx.doi.org/10.1039/c1an15180k

ISSN (print)

00032654

ISSN (electronic)

13645528

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