Document Type
Journal Article
Department/Unit
Department of Chemistry
Title
Preparation and evaluation of a phenylboronate affinity monolith for selective capture of glycoproteins by capillary liquid chromatography
Language
English
Abstract
A phenylboronate affinity monolith was prepared and applied to the selective capture of glycoproteins from unfractionated protein mixtures. The monolith was synthesized in a 100 μm i.d capillary by an in situ polymerization procedure using a pre-polymerization mixture consisting of 4-vinylphenylboronic acid (VPBA) as functional monomer, ethylene dimethacrylate (EDMA) as crosslinker, diethylene glycol and ethylene glycol as binary porogenic solvents, and azobisisobutyronitrile (AIBN) as initiator. The prepared monolith was characterized in terms of the morphology, pore property, and recognition property. The selectivity and dynamic binding capacity were evaluated by using standard glycoproteins and nonglycoproteins as model proteins. The chromatographic results demonstrated that the phenylboronate affinity monolith had higher selectivity and binding capacity for glycoprotein than nonglycoprotein. The resulting phenylboronate affinity monolith was used as the sorbent for in-tube solid phase microextraction (in-tube SPME), and the extraction performance of the monolith was assessed by capture of ovalbumin from egg white sample. © 2011 The Royal Society of Chemistry.
Publication Date
2011
Source Publication Title
Analyst
Volume
136
Issue
16
Start Page
3281
End Page
3288
Publisher
Royal Society of Chemistry
DOI
10.1039/c1an15180k
Link to Publisher's Edition
ISSN (print)
00032654
ISSN (electronic)
13645528
Recommended Citation
Lin, Zi An, Ji Lei Pang, Yao Lin, Hui Huang, Zong Wei Cai, Lan Zhang, and Guo Nan Chen. "Preparation and evaluation of a phenylboronate affinity monolith for selective capture of glycoproteins by capillary liquid chromatography." Analyst 136.16 (2011): 3281-3288.