Document Type

Journal Article

Department/Unit

School of Chinese Medicine; Department of Chemistry

Language

English

Abstract

A crude polysaccharide fraction (cDOP) has been determined to be the characteristic marker of Dendrobium officinale, an expensive tea material in Asia, but its chemistry and bioactivity have not been studied. In work reported here, cDOP was destarched (DOP, 90% yield) and separated into two subfraction polysaccharides, DOPa and DOPb, which were characterized by monosaccharide composition and methylation analyses and spectral analyses (FT-IR and 1H and 13C NMR). Both are composed of mannose and glucose at similar ratios and have a similar structure with a backbone of 1,4-linked β-d-mannopyranosyl and β-d-glucopyranosyl residues. Significant differences were observed only in their molecular weights. Bioassay using mouse macrophage cell line RAW264.7 indicated that DOP and its two subfractions enhance cell proliferation, TNF-α secretion, and phagocytosis in a dose-dependent manner. They also induced the proliferation of lymphocytes alone and with mitogens. DOPa and DOPb are thus proven to be major, active polysaccharide markers of D. officinale.

Keywords

chemical structure, Dendrobium officinale, lymphocytes, polysaccharides, RAW264.7 cells

Publication Date

2016

Source Publication Title

Journal of Agricultural and Food Chemistry

Volume

64

Issue

4

Start Page

881

End Page

889

Publisher

American Chemical Society

Peer Reviewed

1

DOI

10.1021/acs.jafc.5b05180

Link to Publisher's Edition

http://dx.doi.org/10.1021/acs.jafc.5b05180

ISSN (print)

00218561

ISSN (electronic)

15205118

ESSN

15205118

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