School of Chinese Medicine
A tutorial review for employing enzymes for the construction of G-quadruplex-based sensing platforms
With rapid advances in the field of DNA chemistry, nucleic acids and DNA-modifying enzymes have recently emerged as versatile components for the construction of oligonucleotide-based sensors. Meanwhile, the G-quadruplex motif has been widely employed for the development of DNA-based assays due to its diverse structural variety. In this tutorial, we introduce the principles of G-quadruplex-based sensing and the use of DNA-modifying enzymes for sensor platform development. We also highlight recent studies of the application of DNA-modifying enzymes for the development of G-quadruplex-based luminescent detection platforms with a view towards how those enzymes play an important role in sensitivity enhancement.
Oligonucleotide-based sensor, G-quadruplex, Enzyme, Sensor construction
Source Publication Title
Analytica Chimica Acta
Link to Publisher's Edition
Ma, Dik Lung, Wanhe Wang, Zhifeng Mao, Chao Yang, Xiu-Ping Chen, Jin-Jian Lu, Quanbin Han, and Chung-Hang Leung. "A tutorial review for employing enzymes for the construction of G-quadruplex-based sensing platforms." Analytica Chimica Acta 913 (2016): 41-54.