Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

A tutorial review for employing enzymes for the construction of G-quadruplex-based sensing platforms

Language

English

Abstract

With rapid advances in the field of DNA chemistry, nucleic acids and DNA-modifying enzymes have recently emerged as versatile components for the construction of oligonucleotide-based sensors. Meanwhile, the G-quadruplex motif has been widely employed for the development of DNA-based assays due to its diverse structural variety. In this tutorial, we introduce the principles of G-quadruplex-based sensing and the use of DNA-modifying enzymes for sensor platform development. We also highlight recent studies of the application of DNA-modifying enzymes for the development of G-quadruplex-based luminescent detection platforms with a view towards how those enzymes play an important role in sensitivity enhancement.

Keywords

Oligonucleotide-based sensor, G-quadruplex, Enzyme, Sensor construction

Publication Date

3-31-2016

Source Publication Title

Analytica Chimica Acta

Volume

913

Start Page

41

End Page

54

Publisher

Elsevier Masson

Peer Reviewed

1

DOI

10.1016/j.aca.2016.01.043

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.aca.2016.01.043

ISSN (print)

00032670

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