School of Chinese Medicine
Huang-Lian-Jie-Du-Tang (HLJDT), comprising Coptidis Rhizoma, Scutellariae Radix, Phellodendri Cortex and Gardeniae Fructus, is one of the commonly used Chinese medicine formulas for clearing heat and detoxifying. Quality control of the herbal complex like Chinese medicine formulas still remains a challenge. The successful approval of botanical drug Veregen by FDA indicated the importance of quantitative analysis in quality control of herbal medicines. In this study, an effective quantitative method based on conventional HPLC–DAD was developed for simultaneous determination of fourteen major ingredients (seven alkaloids, four flavonoids, three terpenes) in HLJDT. The established method was well validated in terms of linearity, sensitivity, precision, accuracy and stability and then successfully applied to quality evaluation of commercial HLJDT samples. The developed method can quantitatively determine up to 70% of the chemicals of commercial HLJDT sample and effectively revealed the significant variation in the quality of the commercial HLJDT samples collected from different locations.
Journal of Pharmaceutical and Biomedical Analysis
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This study was funded by Hong Kong Baptist University (FRG2/11-12/048, FRG1/12-13/018, FRG2/12-13/006, and RC-start up grant).
Link to Publisher’s Edition
Huang-Lian-Jie-Du-Tang, Chinese medicine formula, Quantitative analysis, Quality evaluation, Simultaneous determination
Kwok, Ka-Yan, Jun Xu, Hing-Man Ho, Hubiao Chen, Min Li, Yan Lang, and Quan-Bin Han. "Quality evaluation of commercial Huang-Lian-Jie-Du-Tang based on simultaneous determination of fourteen major chemical constituents using high performance liquid chromatography." Journal of Pharmaceutical and Biomedical Analysis 85 (2013): 239-244.