Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Language

English

Abstract

Huang-Lian-Jie-Du-Tang (HLJDT), comprising Coptidis Rhizoma, Scutellariae Radix, Phellodendri Cortex and Gardeniae Fructus, is one of the commonly used Chinese medicine formulas for clearing heat and detoxifying. Quality control of the herbal complex like Chinese medicine formulas still remains a challenge. The successful approval of botanical drug Veregen by FDA indicated the importance of quantitative analysis in quality control of herbal medicines. In this study, an effective quantitative method based on conventional HPLC–DAD was developed for simultaneous determination of fourteen major ingredients (seven alkaloids, four flavonoids, three terpenes) in HLJDT. The established method was well validated in terms of linearity, sensitivity, precision, accuracy and stability and then successfully applied to quality evaluation of commercial HLJDT samples. The developed method can quantitatively determine up to 70% of the chemicals of commercial HLJDT sample and effectively revealed the significant variation in the quality of the commercial HLJDT samples collected from different locations.

Keywords

Huang-Lian-Jie-Du-Tang, Chinese medicine formula, Quantitative analysis, Quality evaluation, Simultaneous determination

Publication Date

2013

Source Publication Title

Journal of Pharmaceutical and Biomedical Analysis

Volume

85

Start Page

239

End Page

244

Publisher

Elsevier

Peer Reviewed

1

Funder

This study was funded by Hong Kong Baptist University (FRG2/11-12/048, FRG1/12-13/018, FRG2/12-13/006, and RC-start up grant).

DOI

10.1016/j.jpba.2013.07.033

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.jpba.2013.07.033

ISSN (print)

07317085

ISSN (electronic)

1873264X

ESSN

1873264X

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