Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Language

English

Abstract

A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)– and (1→6)–Glcp, bearing terminal- and (1→3)–Glcp side-chains at O-3 position of (1→6)–Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.

Publication Date

2014

Source Publication Title

PLoS ONE

Volume

9

Issue

7

Start Page

e100380

Publisher

Public Library of Science

Peer Reviewed

1

DOI

10.1371/journal.pone.0100380

Link to Publisher's Edition

http://dx.doi.org/10.1371/journal.pone.0100380

ISSN (electronic)

19326203

ESSN

19326203

Additional Files

JA-5037-28271_publisher_suppl.doc (107 kB)

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