Structural characterization and immuno-modulating activities of a polysaccharide from Ganoderma sinense
School of Chinese Medicine
A protein-bound polysaccharide (GSP-4) with a molecular weight of 8.3 × 105 Da, was isolated from the water extract of the fruiting bodies of Ganoderma sinense. Chemical study revealed that this fraction was composed of mannose, glucose and galactose in the molar ratio of 4.7:27.1:1.0, with the sugar residues of t-, 1,3-, 1,4-, 1,6-, 1,3,4- and 1,3,6-linked Glcp, t-linked Galp, and 1,6-linked Manp. The immnomodulatory effects of GSP-4 were assessed using human peripheral blood mononuclear cells (PBMCs) and murine monocyte/macrophage cell line RAW 264.7. We found that GSP-4 could significantly stimulate the production of the immunomodulatory markers tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-12, and granulocyte-macrophage colony-stimulating factor (GM-CSF) in PBMCs. This observation was further substantiated in RAW 264.7 cells, as indicated by the increase of nitric oxide (NO), TNF-α and IL-6 production. GSP-4 also enhanced the expression of inducible NO synthase mRNA in dose-dependent manner. Our current finding gives the first piece of evidence to support that GSP-4 possesses some promising immunomodulating effects and it could be a potential candidate to be further used in related cancer immunotherapy.
Ganoderma sinense, Polysaccharide, Immunological activities, Human peripheral blood mononuclear cells, Macrophages
Source Publication Title
International Journal of Biological Macromolecules
This research is funded by the Innovation and Technology Fund (ITS/311/09 and InP/108/10) of the Government of the Hong Kong Special Administrative Region and Hong Kong Baptist University (FRG2/11-12/048). The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equipment Grant (SEG CUHK08 and CUHK09) of the University Grants Council of Hong Kong SAR.
Link to Publisher's Edition
Han, X., Chan, B., Yu, H., Yang, Y., Hu, S., Ko, C., Dong, C., Wong, C., Shaw, P., Fung, K., Leung, P., Hsiao, W., Tu, P., & Han, Q. (2012). Structural characterization and immuno-modulating activities of a polysaccharide from Ganoderma sinense. International Journal of Biological Macromolecules, 51 (4). https://doi.org/10.1016/j.ijbiomac.2012.06.029