School of Chinese Medicine
Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense
A polysaccharide (GSP-6B) with a molecular mass of 1.86 × 106 Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-d-glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β-d-glucopyranosyl residues, (1→3)-linked-β-d-glucopyranosyl residues, and nonreducing end β-d-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1β and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.
Journal of Agricultural and Food Chemistry
American Chemical Society
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cytokine, dendritic cell, Ganoderma sinense, immunological activity, polysaccharide
Han, Xiao-Qiang, Ben Chung Lap Chan, Cai-Xia Dong, Yin-Hua Yang, Chun-Hay Ko, Grace Gar-Lee Yue, Dan Chen, Chun-Kwok Wong, Clara Bik-San Lau, Peng-Fei Tu, Pang-Chui Shaw, Kwok-Pui Fung, Ping-Chung Leung, Wen-Luan Hsiao, and Quan-Bin Han. "Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense." Journal of Agricultural and Food Chemistry 60.17 (2012): 4276-4281.