Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense

Language

English

Abstract

A polysaccharide (GSP-6B) with a molecular mass of 1.86 × 106 Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-d-glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β-d-glucopyranosyl residues, (1→3)-linked-β-d-glucopyranosyl residues, and nonreducing end β-d-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1β and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.

Keywords

cytokine, dendritic cell, Ganoderma sinense, immunological activity, polysaccharide

Publication Date

2012

Source Publication Title

Journal of Agricultural and Food Chemistry

Volume

60

Issue

17

Start Page

4276

End Page

4281

Publisher

American Chemical Society

Peer Reviewed

1

DOI

10.1021/jf205056u

Link to Publisher's Edition

http://dx.doi.org/10.1021/jf205056u

ISSN (print)

00218561

ISSN (electronic)

15205118

ESSN

15205118

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