School of Chinese Medicine
Background: Eruberin A (2, 3-dehydroflavonoid), a flavanol glycoside isolated from Pronephrium penangianum, has been used as a blood-nourishing folk medicine for centuries; however, it indeed possesses a variety of other health-promoting benefits including antifibrotic bioactivity. Activation of pancreatic stellate cells (PSCs) is the key initiating step in pancreatic fibrosis, which is a characteristic feature associated with chronic pancreatitis and pancreatic adenocarcinoma. Methods: The anti-fibrotic effect of eruberin A and the underlying mechanisms of its anti-fibrotic action in LTC-14 cells, which retained essential characteristics and morphological features of primary PSCs, were examined by means of real-time polymerase chain reactions, Western blotting and immunostaining. Results: The application of eruberin A (20 μg/ml) effectively inhibited the expression levels of fibrotic mediators namely alphasmooth muscle actin, fibronectin and type I-collagen, so as the sonic hedgehog signaling pathway components post transforming growth factor-beta (5 ng/ml) stimulation. Eruberin A treatment also led to a notable decrease in the activation of nuclear factor-kappaB (NF-κB) and the phosphorylation of phosphoinositide 3-kinase (PI3K)/serine-threonine kinase (AKT). Conclusion: Our results demonstrated that eruberin A significantly suppressed the expression levels of fibrotic mediators in PSCs, and we suggest that its anti-fibrotic mechanism was associated with an attenuation of the PI3K/AKT/NF-κB signaling pathway.
Alpha-smooth muscle actin, Nuclear factor-kappaB, Pancreatic fibrosis, Pancreatic stellate cells, PI3K/AKT, Sonic hedgehog
Source Publication Title
Cellular Physiology and Biochemistry
Karger Publishers Open Access
This is an Open Access article licensed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported license (CC BY-NC) (www.karger.com/OA-license), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only.
This work was supported by the National Natural Science Foundation of China (Young Scientist Project #81400666) and Hong Kong Baptist University (Interdisciplinary Research Matching Scheme #RC-IRMS/12-13/03).
Link to Publisher's Edition
Tsang, S., Zhang, H., Chen, Y., Auyeung, K., & Bian, Z. (2015). Eruberin A, a natural flavanol glycoside, exerts anti-fibrotic action on pancreatic stellate cells. Cellular Physiology and Biochemistry, 6 (36). https://doi.org/10.1159/000430204