School of Chinese Medicine
The root of Panax quinquefolium L., famous as American ginseng all over the world, is one of the most widely-used medicinal or edible materials. Ginsenosides are recognized as the main bioactive chemical components responsible for various functions of American ginseng. In this study, tissue-specific chemicals of P. quinquefolium were analyzed by laser microdissection and ultra-high performance liquid chromatography- quadrupole/time-of-flight-mass spectrometry (UHPLC-Q/TOF–MS) to elucidate the distribution pattern of ginsenosides in tissues. The contents of ginsenosides in various tissues were also compared.
A total of 34 peaks were identified or temporarily identified in the chromatograms of tissue extractions. The cork, primary xylem or cortex contained higher contents of ginsenosides than phloem, secondary xylem and cambium. Thus, it would be reasonable to deduce that the ratio of total areas of cork, primary xylem and the cortex to the area of the whole transection could help to judge the quality of American ginseng by microscopic characteristics.
ConclusionThis study sheds new light on the role of microscopic research in quality evaluation, and provides useful information for probing the biochemical pathways of ginsenosides.
Chemistry Central Journal
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This work is sponsored by the Faculty Research Grant of Hong Kong Baptist University (FRG2/12-13/030) and Innovation and Technology Fund (ITS/185/13FX). We are grateful to Mr. Alan Ho from the School of Chinese Medicine, Hong Kong Baptist University for his technical support.
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© Chen et al. 2015. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Ginsenosides, Laser microdissection, Panax quinquefolium L., Tissue-specific, UHPLC-Q/TOF-MS
Chen, Yujie, Liang Xu, Yuancen Zhao, Zhongzhen Zhao, Hubiao Chen, Tao Yi, Minjian Qin, and Zhitao Liang. "Tissue-specific metabolite profiling and quantitative analysis of ginsenosides in Panax quinquefolium using laser microdissection and liquid chromatography–quadrupole/time of flight-mass spectrometry." Chemistry Central Journal 9 (2015): 66-13.