Document Type
Journal Article
Title
Anti-inflammatory actions of (+)-3′α-angeloxy-4′-keto-3′,4′-dihydroseselin (Pd-Ib) against dextran sulfate sodium-induced colitis in C57BL/6 mice
Department/Unit
School of Chinese Medicine
Language
English
Abstract
The immunoregulatory protective properties of (+)-3′α-angeloxy-4′-keto-3′,4′-dihydroseselin (Pd-Ib) isolated from Bupleurum malconense has not been reported. In the present study, the therapeutic effect of Pd-Ib (30, 60, and 120 mg/kg/day) was examined in a mouse model of dextran sulfate sodium (DSS)-induced acute colitis. Administration of Pd-Ib significantly reduced the disease activity index, inhibited the shortening of colon length, reduced colonic tissue damage, and suppressed colonic myeloperoxidase activity and nitric oxide levels in mice with DSS-induced colitis. Moreover, Pd-Ib greatly suppressed the secretion of pro-inflammatory cytokines TNF-α, IFN-γ, IL-6, and IL-17A while enhancing the level of anti-inflammatory cytokine IL-4. The protein levels of phosphorylated STAT3 (p-STAT3) and phosphorylated p38 (p-p38) were down-regulated in the colonic tissues of DSS-treated mice. Importantly, the anti-inflammatory effect of Pd-Ib against acute colitis was comparable to the anti-inflammatory sulfa drug sulfasalazine (300 mg/kg). Furthermore, the in vitro study showed that the inhibitory effect of Pd-Ib on p-STAT3 and IL-6 protein levels was accompanied by the reduction of MAPKs (JNK and p38). In conclusion, this study suggested that Pd-Ib attenuated DSS-induced acute colitis via the regulation of interleukins principally through the STAT3 and MAPK pathways.
Publication Date
4-30-2016
Source Publication Title
Journal of Natural Products
Volume
79
Issue
4
Start Page
1056
End Page
1062
Publisher
American Chemical Society
Peer Reviewed
1
DOI
10.1021/acs.jnatprod.5b01071
Link to Publisher's Edition
http://dx.doi.org/10.1021/acs.jnatprod.5b01071
ISSN (print)
01633864
APA Citation
Mu, H., Liu, J., Fatima, S., Lin, C., Shi, X., Du, B., Xiao, H., Fan, B., & Bian, Z. (2016). Anti-inflammatory actions of (+)-3′α-angeloxy-4′-keto-3′,4′-dihydroseselin (Pd-Ib) against dextran sulfate sodium-induced colitis in C57BL/6 mice. Journal of Natural Products, 79 (4). https://doi.org/10.1021/acs.jnatprod.5b01071