Anti-inflammatory actions of (+)-3′α-angeloxy-4′-keto-3′,4′-dihydroseselin (Pd-Ib) against dextran sulfate sodium-induced colitis in C57BL/6 mice
School of Chinese Medicine
The immunoregulatory protective properties of (+)-3′α-angeloxy-4′-keto-3′,4′-dihydroseselin (Pd-Ib) isolated from Bupleurum malconense has not been reported. In the present study, the therapeutic effect of Pd-Ib (30, 60, and 120 mg/kg/day) was examined in a mouse model of dextran sulfate sodium (DSS)-induced acute colitis. Administration of Pd-Ib significantly reduced the disease activity index, inhibited the shortening of colon length, reduced colonic tissue damage, and suppressed colonic myeloperoxidase activity and nitric oxide levels in mice with DSS-induced colitis. Moreover, Pd-Ib greatly suppressed the secretion of pro-inflammatory cytokines TNF-α, IFN-γ, IL-6, and IL-17A while enhancing the level of anti-inflammatory cytokine IL-4. The protein levels of phosphorylated STAT3 (p-STAT3) and phosphorylated p38 (p-p38) were down-regulated in the colonic tissues of DSS-treated mice. Importantly, the anti-inflammatory effect of Pd-Ib against acute colitis was comparable to the anti-inflammatory sulfa drug sulfasalazine (300 mg/kg). Furthermore, the in vitro study showed that the inhibitory effect of Pd-Ib on p-STAT3 and IL-6 protein levels was accompanied by the reduction of MAPKs (JNK and p38). In conclusion, this study suggested that Pd-Ib attenuated DSS-induced acute colitis via the regulation of interleukins principally through the STAT3 and MAPK pathways.
Source Publication Title
Journal of Natural Products
American Chemical Society
Link to Publisher's Edition
Mu, H., Liu, J., Fatima, S., Lin, C., Shi, X., Du, B., Xiao, H., Fan, B., & Bian, Z. (2016). Anti-inflammatory actions of (+)-3′α-angeloxy-4′-keto-3′,4′-dihydroseselin (Pd-Ib) against dextran sulfate sodium-induced colitis in C57BL/6 mice. Journal of Natural Products, 79 (4). https://doi.org/10.1021/acs.jnatprod.5b01071